Summary: Directed biomolecular evolution is widely used to tailor and enhance enzymes, fluorescent proteins, and antibodies but has hitherto not been applied in the reprogramming of mammalian cells.Here, we describe a method termed directed evolution of reprogramming factors by cell selection and sequencing (DERBY-seq) to identify artificially enha
Gallbladder carcinoma can be challenging Vitamin K to diagnose and treat and usually leads to poor outcome, due to its aggressive nature and the nonspecific clinical presentation at early stage.We describe an interesting case of a 60-year-old female who presented with stage 3 appendiceal carcinoma after appendectomy was performed outside hospital.F
BackgroundHuman immunodeficiency virus (HIV) is responsible for millions of deaths every year.The current treatment involves the use of multiple antiretroviral agents that may harm patients due to their toxic nature.RNA interference (RNAi) is a potent candidate for the future treatment of HIV, uses short interfering RNA (siRNA/shRNA) for silencing
Following recent successful results in the search for innovative semi-synthetic cosmeceutical Se-glycoconjugates, the work reported herein explores the development and evaluation of second-generation selenosugar-linked hydroxycinnamic acids as new potential cosmeceutical ingredients.Utilizing a Pummerer-like rearrangement for C1-acetylation, these
Accessories This article examines the jurisdictional status of the internet.Because of the underly ing nature of technology, nations cannot effectively monitor online transactions that originate or conclude within their borders.Governments can seek to enforce their laws within the constraints of their physical, geographical, and political domains a